PROSCALE

Scalable microbial protein ingredients for appealing nutritious foods

Project details

Type of project
Innovation Action - Demonstration
Feedstock origin
Sidestreams from industry
Feedstock type
Food industry sidestreams
Project period
1 September 2026 - 31 August 2030
Status
Upcoming
CBE JU Contribution
€ 6 992 742,50
Call identifier
HORIZON-JU-CBE-2025

Summary

Recent global geopolitical challenges highlight the importance of diversifying protein sources for food security and sustainability. Reducing dependence on animal proteins and the global reliance on four major crops - maize, soy, rice and wheat - through the development of alternatives such as single-cell proteins is essential for food security. Single-cell proteins also offer a more resource-efficient and climate-friendly alternative to conventional animal proteins.  

PROSCALE aims to advance the production of high-quality alternative proteins for food applications by scaling up continuous fermentation processes for fungal, yeast, and bacterial single-cell proteins. The project uses food industry sidestreams, such as processing residues from potato, wheat, and vegetables, as well as by-products from baking and pasta production, as sustainable fermentation feedstocks.  

The project combines upstream and downstream process innovation with a digital twin platform to optimise resource efficiency, reduce environmental impacts, and ensure scalable, safe, multifunctional protein ingredients (>50% protein dry matter). These include fungal mycoproteins, yeast proteins, and bacterial proteins for applications such as meat and dairy substitutes, sports and health-conscious nutrition, and staple foods like bread and pasta.  

In addition, by applying a cascaded, minimal-waste approach, co-products are reintegrated into the production cycle or transformed into non-food applications, including the conversion of side-streams into methane and CO₂-derived compounds, as well as the development of palatants for pet food applications.  

  • Advance sustainable, efficient, and safe protein production for food application via continuous biomass fermentation, using locally sourced substrates derived from food industry sidestreams. 
  • Provide a competitive advantage compared to similar products on the market in terms of production costs, improved taste, and validated sensory acceptance. 
  • Enable cost-effective fermentation substrates derived from food industry sidestreams, developed by applying innovative methods to remove or detoxify growth inhibitors, eliminate and/or transform non-fermentable suspended solids, as well as optimise substrate formulation.  
  • Demonstrate the versatility and applicability of single-cell protein in enhancing palatability, nutritional adequacy, and in vitro digestibility across food applications, with critical quality parameters established to support large-scale production. 
  • Develop a more circular and zero-waste approach for cascaded use of project co-products through water and spent medium recycling, CO₂-to-C2/C3 conversion, and pet food applications, while ensuring measures to minimise toxicological risks or reintroduction of pathogens and contaminants.  
  • Evaluate and optimise the resource efficiency of PROSCALE processes, targeting at least a 40% reduction in climate-change impact and lower waste generation.  
  • Develop business models and value proposition strategies to enhance future market entry and consumer acceptance of the project ingredients and food applications. 
  • Implement and enhance a multi-actor approach through effective communication and dissemination of project outcomes and policy briefs, fostering synergies with related initiatives, stakeholder co-creation, and societal acceptance of single-cell proteins, ensuring targeted outreach.  

  • Increase EU companies’ revenue by approximately €850million through the projected capture of up to 10% of the single-cell protein market and licensing advanced feedstock transformation know-how. 
  • Improve resource efficiency by 40–60% through the optimised use of underutilised sidestreams from food and feed production. 
  • Contribute to improving climate neutrality by 20% across PROSCALE processes.  
  • Boost the circularity of project solutions by 2-7% by using co-products for pet food or technical applications.  
  • Target a tenfold increase in fermenter capacity, supporting the long-term potential to produce significant quantities of dietary protein for the EU population. 
  • Diversify protein sources and production systems to strengthen resilience against environmental or geopolitical crises. 
  • Projected reduction greenhouse gas emissions and water consumption by 60% compared to traditional protein source production, while minimising waste generation through new feedstock transformation processes and optimised value chains. 

Consortium map

Project coordination

  • TEKNOLOGIAN TUTKIMUSKESKUS VTT OY Espoo, Finland

Consortium

  • CIRCE BIOTECHNOLOGIE GMBH Wien, Austria
  • VAASAN YLIOPISTO Vaasa, Finland
  • KUBARA SPOLKA Z OGRANICZONA ODPOWIEDZIALNOSCIA Czestochowa, Poland
  • AMERIKANIKO KOLLEGIO ELLADOS, KENTRO EREVNAS AGIA PARASKEVI, Greece
  • SINONIN BIOTECH GmbH LANGWEDEL, Germany
  • EIT FOOD HEVERLEE, Belgium
  • TETRA PAK PROCESSING SYSTEMS AKTIEBOLAG Lund, Sweden
  • DIL DEUTSCHES INSTITUT FUR LEBENSMITTELTECHNIK EV Quakenbruck, Germany
  • ENIFERBIO OY Espoo, Finland
  • BARILLA G. E R. FRATELLI SPA Parma, Italy
  • CENTRE DE RECHERCHE DE L'INSTITUT LYFE ECULLY, France
  • STICHTING EFFOST Wageningen, Netherlands
  • DUYNIE HOLDING BV Alphen Aan Den Rijn, Netherlands
  • PINAR SUT MAMULLERI SANAYI VE TICARET A.S. İzmir, Türkiye
  • STICHTING WAGENINGEN RESEARCH Wageningen, Netherlands